Total amount of contrast did not exceed 350 ml in patients Galardin in vitro with GFR 45-59 ml/min/1.73 m(2) and 250 ml – with GFR

<45 ml/min/1.73 m(2). In all patients with GFR >= 60 ml/min/1.73 m(2) low osmolar contrast preparations were used (total amount – less then 600 ml per patient). Immediate success of PCI was similar in all groups (994%, 98.2% 97.4%, respectively). Rate of CIN rose significantly in groups 2 and 3(0.4%, 4.9%, 13.2%, respectively, p<0.001). Before 6 months after PCI restenosis developed more frequently with lowering of GFR (group 1 – 11%, group 2 – 22%, group 3 – 34%, p<0.001). Myocardial infarction developed by 3 years in 6, 10, and 26% of patients in groups 1, 2, and 3, respectively. Lethality during 3 years was 5, 10, and 24% in groups 1, 2, and 3, respectively. Regression analysis showed that as a whole 3 years rate of myocardial infarction rose 1.57 times in group 2 compared with group 1, and 3.91 times in group 3 compared with group 1. Mortality by 3 years rose 1.93 times in group 2 compared with group 1, and 4.52 times in group 3 compared with group 1. Thus, presence of initially

lowered GFR increases risk of CIN after 3-MA nmr elective implantations of standard stents, leads to rise of restenosis rate by 6 months and increase of mortality and rate of nonfatal myocardial infarction by 3 years.”
“Telomere length was sequentially determined in peripheral blood leukocytes (PBL) from patients with anlcylosing spondylitis (AS; n = 44) and psoriatic arthritis (PsA; n = 42) followed

through 2.93 +/- 0.99 years, using a quantitative PCR (qPCR) assay. The initial telomere size from PsA patients was higher than those with cutaneous psoriasis only (n=53), possibly due to the inflammatory condition. The gPCR assay was sensitive enough to evidence a significant telomere length shortening in PBL from practically all subjects and PsA patients showed a higher rate of loss of telomere sequence than patients with AS during the follow-up time. (C) 2014 Elsevier B.V. All rights reserved.”
“Preparations of Na,K-ATPase from outer medulla of rabbit kidney purified in accordance with the method of P. L. Jorgensen were shown to contain as admixture a protease that moves with alpha-subunit (similar to BMS-777607 100 kDa) as a single protein band during one-dimensional SDS-PAGE. The electro-elution of proteins of this band from polyacrylamide gel results in the appearance of two protein fragments (similar to 67 and 55 kDa) that are stained with polyclonal antibodies against Na,K-ATPase alpha-subunit. Liquid chromatography/tandem mass spectrometry (LC/MS/MS) analysis showed that the neutral membrane-bound endopeptidase neprilysin is located in one protein band together with the Na,K-ATPase alpha-subunit. Addition of thiorphan, a specific inhibitor of neutral endopeptidase, eliminates proteolysis of the alpha-subunit. The data demonstrate that Na,K-ATPase alpha-subunit may be a natural target for neprilysin.